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KMID : 1143420190120341260
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Public Health Weekly Report
2019 Volume.12 No. 34 p.1260 ~ p.1266
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Laboratory-based diagnosis of Japanese encephalitis in Korea, 2018
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Lee Hyeok-Jin
Lee Eun-Ju
Choi Woo-Young
Han Myung-Guk
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Abstract
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Japanese encephalitis virus (JEV), a mosquito-borne zoonotic pathogen, is one of the major causes of viral encephalitis. JEV is categorized into five genotypes (genotypes I, II, III, IV, and V), based on the nucleotide sequence of the envelope gene, and three genotypes (I, III, and V) of JEV were confirmed in Korea. Laboratory testing for JEV was conducted using real-time, IgM ELISA, indirect immunofluorescence assay (IFA), plaque reduction neutralization test (PRNT), and virus isolation in cell culture in serum and cerebrospinal fluid. A total of 17 JEV patients were confirmed by serological and molecular tests in 2018. Two cases of JEV were detected by real-time RT-PCR and were found to be of genotype V of JEV. We also isolated JEV in LLCMK2 cells from the cerebrospinal fluid of an encephalitis patient in 2018. The complete nucleotide and amino acid sequences of the isolate have been determined. We infer from our data that genotype V of JEV is circulating in Korea.
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KEYWORD
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Japanese encephalitis, Japanese encephalitis virus, Genotype V, Virus isolation
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